Next generation sequencing-based analysis of repetitive DNA in the model dioecious [corrected] plant Silene latifolia.

ABSTRACT:

Background

Silene latifolia is a dioecious [corrected] plant with well distinguished X and Y chromosomes that is used as a model to study sex determination and sex chromosome evolution in plants. However, efficient utilization of this species has been hampered by the lack of large-scale sequencing resources and detailed analysis of its genome composition, especially with respect to repetitive DNA, which makes up the majority of the genome.

Methodology/principal findings

We performed low-pass 454 sequencing followed by similarity-based clustering of 454 reads in order to identify and characterize sequences of all major groups of S. latifolia repeats. Illumina sequencing data from male and female genomes were also generated and employed to quantify the genomic proportions of i

SUBMITTER: Macas J 

PROVIDER: S-EPMC3212565 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature