Nitrous oxide reductase (nosZ) gene fragments differ between native and cultivated Michigan soils.

ABSTRACT: The effect of standard agricultural management on the genetic heterogeneity of nitrous oxide reductase (nosZ) fragments from denitrifying prokaryotes in native and cultivated soil was explored. Thirty-six soil cores were composited from each of the two soil management conditions. nosZ gene fragments were amplified from triplicate samples, and PCR products were cloned and screened by restriction fragment length polymorphism (RFLP). The total nosZ RFLP profiles increased in similarity with soil sample size until triplicate 3-g samples produced visually identical RFLP profiles for each treatment. Large differences in total nosZ profiles were observed between the native and cultivated soils. The fragments representing major groups of clones encountered at least twice and four randomly selected

SUBMITTER: Stres B 

PROVIDER: S-EPMC321260 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature