Whole genome analysis of linezolid resistance in Streptococcus pneumoniae reveals resistance and compensatory mutations.

ABSTRACT:

Background

Several mutations were present in the genome of Streptococcus pneumoniae linezolid-resistant strains but the role of several of these mutations had not been experimentally tested. To analyze the role of these mutations, we reconstituted resistance by serial whole genome transformation of a novel resistant isolate into two strains with sensitive background. We sequenced the parent mutant and two independent transformants exhibiting similar minimum inhibitory concentration to linezolid.

Results

Comparative genomic analyses revealed that transformants acquired G2576T transversions in every gene copy of 23S rRNA and that the number of altered copies correlated with the level of linezolid resistance and cross-resistance to florfenicol and chloramphenicol. One of the tr

SUBMITTER: Billal DS 

PROVIDER: S-EPMC3212830 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature