A survey of small RNAs in human sperm.

ABSTRACT:

Background

There has been substantial interest in assessing whether RNAs (mRNAs and sncRNAs, i.e. small non-coding) delivered from mammalian spermatozoa play a functional role in early embryo development. While the cadre of spermatozoal mRNAs has been characterized, comparatively little is known about the distribution or function of the estimated 24,000 sncRNAs within each normal human spermatozoon.

Methods

RNAs of <200 bases in length were isolated from the ejaculates from three donors of proved fertility. RNAs of 18-30 nucleotides in length were then used to construct small RNA Digital Gene Expression libraries for Next Generation Sequencing. Known sncRNAs that uniquely mapped to a single location in the human genome were identified.

Results

Bioinformatic analysis r

SUBMITTER: Krawetz SA 

PROVIDER: S-EPMC3212879 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature