Separating spermatogonia from cancer cells in contaminated prepubertal primate testis cell suspensions.

ABSTRACT:

Background

Chemotherapy and radiation treatments for cancer and other diseases can cause male infertility. There are currently no options to preserve the fertility of prepubertal boys who are not yet making sperm. Cryopreservation of spermatogonial stem cells (SSCs, obtained via testicular biopsy) followed by autologous transplantation back into the testes at a later date may restore fertility in these patients. However, this approach carries an inherent risk of reintroducing cancer.

Methods

To address this aspect of SSC transplantation safety, prepubertal non-human primate testis cell suspensions were inoculated with MOLT4 T-lymphoblastic leukemia cells and subsequently sorted for cell surface markers CD90 (THY-1) and CD45.

Results

Cancer cells segregated to the CD90

SUBMITTER: Hermann BP 

PROVIDER: S-EPMC3212882 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature