Overexpression of hepatoma-derived growth factor in melanocytes does not lead to oncogenic transformation.

ABSTRACT:

Background

HDGF is a growth factor which is overexpressed in a wide range of tumors. Importantly, expression levels were identified as a prognostic marker in some types of cancer such as melanoma.

Methods

To investigate the presumed oncogenic/transforming capacity of HDGF, we generated transgenic mice overexpressing HDGF in melanocytes. These mice were bred with mice heterozygous for a defective copy of the Ink4a tumor suppressor gene and were exposed to UV light to increase the risk for tumor development both genetically and physiochemically. Mice were analyzed by immunohistochemistry and Western blotting. Furthermore, primary melanocytes were isolated from different strains created.

Results

Transgenic animals overexpressed HDGF in hair follicle melanocytes. Interest

SUBMITTER: Sedlmaier A 

PROVIDER: S-EPMC3213223 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature