Dataset Information

Isolation and Characterization of Chicken NPAS3.

ABSTRACT: Here we describe characterization of chicken neuronal Per-Arnt-Sim domain 3 (NPAS3) gene during embryogenesis including examinations of expression pattern and function of the gene. RTPCR assay showed that the primary tissue of expression for this gene is the central nervous system (CNS) while RNA in situ hybridization assay confirmed that NPAS3 was expressed in the ventricular zone of developing neural tube as early as Hamburger-Hamilton (HH) stage 20. Ectopic over-expression of the gene in ovo in the developing chicken neural tube by electroporation had little effect on stem cell population, overall neurogenesis, and motor neuron differentiation. We discuss the implications of our observation.

SUBMITTER: Shin J

PROVIDER: S-EPMC3214776 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature

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Isolation and Characterization of Chicken NPAS3.

Shin Jiheon J Jeong Hye Yun HY Lee Kyung Eun KE Kim Jaesang J

Experimental neurobiology 20100930 2

Here we describe characterization of chicken neuronal Per-Arnt-Sim domain 3 (NPAS3) gene during embryogenesis including examinations of expression pattern and function of the gene. RTPCR assay showed that the primary tissue of expression for this gene is the central nervous system (CNS) while RNA in situ hybridization assay confirmed that NPAS3 was expressed in the ventricular zone of developing neural tube as early as Hamburger-Hamilton (HH) stage 20. Ectopic over-expression of the gene in ovo ...[more]

PMID: 22110344

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Dataset Information

Isolation and Characterization of Chicken NPAS3.

Publications

Isolation and Characterization of Chicken NPAS3.

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