An in silico approach combined with in vivo experiments enables the identification of a new protein whose overexpression can compensate for specific respiratory defects in Saccharomyces cerevisiae.

ABSTRACT:

Background

The mitochondrial inner membrane contains five large complexes that are essential for oxidative phosphorylation. Although the structure and the catalytic mechanisms of the respiratory complexes have been progressively established, their biogenesis is far from being fully understood. Very few complex III assembly factors have been identified so far. It is probable that more factors are needed for the assembly of a functional complex, but that the genetic approaches used to date have not been able to identify them. We have developed a systems biology approach to identify new factors controlling complex III biogenesis.

Results

We collected all the physical protein-protein interactions (PPI) involving the core subunits, the supernumerary subunits and the assembly fact

SUBMITTER: Glatigny A 

PROVIDER: S-EPMC3214889 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature