Pancreatic stellate cell models for transcriptional studies of desmoplasia-associated genes.

ABSTRACT:

Background

Pancreatic stellate cells are emerging as key players in pathophysiopathological processes underlying the development of pancreatic disease, including pancreatitis and cancer. The cells are scarce in the pancreas making their isolation time and resource use consuming.

Methods

Therefore, with the ultimate goal of facilitating mechanistic studies, here we report the isolation, characterization, and immortalization of stellate cell lines from rat and mouse origin.

Results

These cell lines display morphological and molecular markers as well as non-tumorigenic characteristics similar to the frequently used hepatic counterparts. In addition, we have tested their robustness as a model for transcriptional regulatory studies. We find that these cells respond well to

SUBMITTER: Mathison A 

PROVIDER: S-EPMC3214918 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature