Identification of the calcitonin receptor in osteoarthritic chondrocytes.

ABSTRACT:

Background

Preclinical and clinical studies have shown that salmon calcitonin has cartilage protective effects in joint degenerative diseases, such as osteoarthritis (OA). However, the presence of the calcitonin receptor (CTR) in articular cartilage chondrocytes is yet to be identified. In this study, we sought to further investigate the expression of the CTR in naïve human OA articular chondrocytes to gain further confirmation of the existents of the CTR in articular cartilage.

Methods

Total RNA was purified from primary chondrocytes from articular cartilage biopsies from four OA patients undergoing total knee replacement. High quality cDNA was produced using a dedicated reverse transcription polymerase chain reaction (RT-PCR) protocol. From this a nested PCR assay amplifyi

SUBMITTER: Segovia-Silvestre T 

PROVIDER: S-EPMC3214920 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature