RNA interference-based resistance against a legume mastrevirus.

ABSTRACT:

Background

RNA interference (RNAi) is a homology-dependant gene silencing mechanism and has been widely used to engineer resistance in plants against RNA viruses. However, its usefulness in delivering resistance against plant DNA viruses belonging to family Geminiviridae is still being debated. Although the RNAi approach has been shown, using a transient assay, to be useful in countering monocotyledonous plant-infecting geminiviruses of the genus Mastrevirus, it has yet to be investigated as a means of delivering resistance to dicot-infecting mastreviruses. Chickpea chlorotic dwarf Pakistan virus (CpCDPKV) is a legume-infecting mastrevirus that affects chickpea and other leguminous crops in Pakistan.

Results

Here a hairpin (hp)RNAi construct containing sequences encompassing

SUBMITTER: Nahid N 

PROVIDER: S-EPMC3214926 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature