Transcriptional signatures of regulatory and toxic responses to benzo-[a]-pyrene exposure.

ABSTRACT:

Background

Small molecule ligands often have multiple effects on the transcriptional program of a cell: they trigger a receptor specific response and additional, indirect responses ("side effects"). Distinguishing those responses is important for understanding side effects of drugs and for elucidating molecular mechanisms of toxic chemicals.

Results

We explored this problem by exposing cells to the environmental contaminant benzo-[a]-pyrene (B[a]P). B[a]P exposure activates the aryl hydrocarbon receptor (Ahr) and causes toxic stress resulting in transcriptional changes that are not regulated through Ahr. We sought to distinguish these two types of responses based on a time course of expression changes measured after B[a]P exposure. Using Random Forest machine learning we cla

SUBMITTER: Michaelson JJ 

PROVIDER: S-EPMC3215681 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature