Cancer associated epigenetic transitions identified by genome-wide histone methylation binding profiles in human colorectal cancer samples and paired normal mucosa.

ABSTRACT:

Background

Despite their well-established functional roles, histone modifications have received less attention than DNA methylation in the cancer field. In order to evaluate their importance in colorectal cancer (CRC), we generated the first genome-wide histone modification profiles in paired normal colon mucosa and tumor samples.

Methods

Chromatin immunoprecipitation and microarray hybridization (ChIP-chip) was used to identify promoters enriched for histone H3 trimethylated on lysine 4 (H3K4me3) and lysine 27 (H3K27me3) in paired normal colon mucosa and tumor samples from two CRC patients and for the CRC cell line HT29.

Results

By comparing histone modification patterns in normal mucosa and tumors, we found that alterations predicted to have major functional consequ

SUBMITTER: Enroth S 

PROVIDER: S-EPMC3216894 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature