Cryo-electron tomography of Marburg virus particles and their morphogenesis within infected cells.

ABSTRACT: Several major human pathogens, including the filoviruses, paramyxoviruses, and rhabdoviruses, package their single-stranded RNA genomes within helical nucleocapsids, which bud through the plasma membrane of the infected cell to release enveloped virions. The virions are often heterogeneous in shape, which makes it difficult to study their structure and assembly mechanisms. We have applied cryo-electron tomography and sub-tomogram averaging methods to derive structures of Marburg virus, a highly pathogenic filovirus, both after release and during assembly within infected cells. The data demonstrate the potential of cryo-electron tomography methods to derive detailed structural information for intermediate steps in biological pathways within intact cells. We describe the location and arrange

SUBMITTER: Bharat TA 

PROVIDER: S-EPMC3217011 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature