Proteomics analysis of chinese hamster ovary cells undergoing apoptosis during prolonged cultivation.

ABSTRACT: The degradation of environmental conditions, such as nutrient depletion and accumulation of toxic waste products over time, often lead to premature apoptotic cell death in mammalian cell cultures and suboptimal protein yield. Although apoptosis has been extensively researched, the changes in the whole cell proteome during prolonged cultivation, where apoptosis is a major mode of cell death, have not been examined. To our knowledge, the work presented here is the first whole cell proteome analysis of non-induced apoptosis in mammalian cells. Flow cytometry analyses of various activated caspases demonstrated the onset of apoptosis in Chinese hamster ovary cells during prolonged cultivation was primarily through the intrinsic pathway. Differential in gel electrophoresis proteomic study compar

SUBMITTER: Wei YY 

PROVIDER: S-EPMC3217071 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature