Molecular phenotype of monocytes at the maternal-fetal interface.

ABSTRACT:

Objective

The purpose of this study was to gain insight into the pathways that are associated with inflammation at the maternal-fetal interface. This study examined the molecular characteristics of monocytes that were derived from the maternal circulation and the placenta of obese women.

Study design

Mononuclear cells were isolated from placenta, venous maternal, and umbilical cord blood at term delivery; activated monocytes were separated with CD14 immunoselection. The genotype and expression pattern of the monocytes were analyzed by microarray and real-time reverse transcriptase-polymerase chain reaction.

Results

The transcriptome of the maternal blood and placental CD14 monocytes exhibited 73% homology, with 10% (1800 common genes) differentially expressed. Genes f

SUBMITTER: Basu S 

PROVIDER: S-EPMC3217185 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature