Do-it-yourself: construction of a custom cDNA macroarray platform with high sensitivity and linear range.

ABSTRACT:

Background

Research involving gene expression profiling and clinical applications, such as diagnostics and prognostics, often require a DNA array platform that is flexibly customisable and cost-effective, but at the same time is highly sensitive and capable of accurately and reproducibly quantifying the transcriptional expression of a vast number of genes over the whole transcriptome dynamic range using low amounts of RNA sample. Hereto, a set of easy-to-implement practical optimisations to the design of cDNA-based nylon macroarrays as well as sample (33)P-labeling, hybridisation protocols and phosphor screen image processing were analysed for macroarray performance.

Results

The here proposed custom macroarray platform had an absolute sensitivity as low as 50,000 transcripts

SUBMITTER: Boonefaes T 

PROVIDER: S-EPMC3217856 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature