Protein release through nonlethal oncotic pores as an alternative nonclassical secretory pathway.

ABSTRACT:

Background

Nonclassical (unconventional) protein secretion is thought to represent the primary secretion mechanism for several cytosolic proteins, such as HIV-Tat, galectin 1, interleukin-1β, and several proteins that shuttle between the nucleus and cytosol, such as fibroblast growth factor 1 (FGF1), FGF2, and nucleolin. Four nonclassical secretory pathways have been described including direct transport (presumably through transporters in the plasma membrane), secretion via exosomes, lysosomal secretion, and blebbing. The purpose of this study was to gain mechanistic insight into nonclassical protein secretion using phosphoglycerate kinase 1 (PGK1), a previously identified nonclassical secretory protein, as a reporter protein.

Results

Upon shifting HeLa cells into serum-free

SUBMITTER: Chirico WJ 

PROVIDER: S-EPMC3217904 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature