Intracellular trafficking of polyamidoamine-poly(ethylene glycol) block copolymers in DNA delivery.

ABSTRACT: The delivery of nucleic acids has the potential to revolutionize medicine by allowing previously untreatable diseases to be clinically addressed. Viral delivery systems have shown immunogenicity and toxicity dangers, but synthetic vectors have lagged in transfection efficiency. Previously, we developed a modular, linear-dendritic block copolymer architecture with high gene transfection efficiency compared to commercial standards. This rationally designed system makes use of a cationic dendritic block to condense the anionic DNA and forms complexes with favorable endosomal escape properties. The linear block provides biocompatibility and protection from serum proteins, and can be functionalized with a targeting ligand. In this work, we quantitate performance of this system with respect to i

SUBMITTER: Bonner DK 

PROVIDER: S-EPMC3218102 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature