Endogenous transcription at the centromere facilitates centromere activity in budding yeast.

ABSTRACT:

Background

The centromere (CEN) DNA-kinetochore complex is the specialized chromatin structure that mediates chromosome attachment to the spindle and is required for high-fidelity chromosome segregation. Although kinetochore function is conserved from budding yeast to humans, it was thought that transcription had no role in centromere function in budding yeast, in contrast to other eukaryotes including fission yeast.

Results

We report here that transcription at the centromere facilitates centromere activity in the budding yeast Saccharomyces cerevisiae. We identified transcripts at CEN DNA and found that Cbf1, which is a transcription factor that binds to CEN DNA, is required for transcription at CEN DNA. Chromosome instability of cbf1Δ cells is suppressed by transcription d

SUBMITTER: Ohkuni K 

PROVIDER: S-EPMC3218120 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature