Calcium-mediated transductive systems and functionally active gap junctions in astrocyte-like GL15 cells.

ABSTRACT:

Background

It has been proposed that GL15, a human cell line derived from glioblastoma multiforme, is a possible astroglial-like cell model, based on the presence of cytoplasmic glial fibrillary acidic protein.

Results

The aim of this work was to delineate the functional characteristics of GL15 cells using various experimental approaches, including the study of morphology, mechanism of induction of intracellular Ca2+ increase by different physiological agonists, and the presence and permeability of the gap-junction system during cell differentiation. Immunostaining experiments showed the presence and localization of specific glial markers, such as glial fibrillary acidic protein and S100B, and the lack of the neuronal marker S100A. Notably, all the Ca2+ pathways present in a

SUBMITTER: Mariggio MA 

PROVIDER: S-EPMC32183 | biostudies-literature | 2001

REPOSITORIES: biostudies-literature