Molecular Characterization of Heat Shock Protein 70-1 Gene of Goat (Capra hircus).

ABSTRACT: Heat shock protein 70 (HSP 70) plays a vital role by bestowing cytoprotection against diverse kinds of stresses. The ubiquitous HSP 70 proteins are the most abundant and temperature sensitive among all the HSPs. The present paper has characterized HSP70-1 cDNA in goat (Capra hircus). Total RNA isolated from goat peripheral blood mononuclear cells was reverse transcribed to cDNA that was used for amplification of HSP 70-1 gene. PCR product (1926 bp) was cloned in pGEM-T easy vector and sequenced. Sequence analysis revealed 1926-bp-long open reading frame of HSP 70-1 gene encoding 641 amino acids in goat, as reported in cattle. At nucleotide level, goat HSP 70-1 was found to be 96-99% similar to that of sheep (partial), cattle, and buffalo whereas the similarity at amino acid level was 95-10

SUBMITTER: Gade N 

PROVIDER: S-EPMC3218308 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature