Recombinant Marburg virus expressing EGFP allows rapid screening of virus growth and real-time visualization of virus spread.

ABSTRACT: The generation of recombinant enhanced green fluorescent protein (EGFP)-expressing viruses has significantly improved the study of their life cycle and opened up the possibility for the rapid screening of antiviral drugs. Here we report rescue of a recombinant Marburg virus (MARV) expressing EGFP from an additional transcription unit (ATU). The ATU was inserted between the second and third genes, encoding VP35 and VP40, respectively. Live-cell imaging was used to follow virus spread in real time. EGFP expression was detected at 32 hours postinfection (hpi), and infection of neighboring cells was monitored at 55 hpi. Compared to the parental virus, production of progeny rMARV-EGFP was reduced 4-fold and lower protein levels of VP40, but not nucleoprotein, were observed, indicating a decreas

SUBMITTER: Schmidt KM 

PROVIDER: S-EPMC3218667 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature