The role of chromatin accessibility in directing the widespread, overlapping patterns of Drosophila transcription factor binding.

ABSTRACT:

Background

In Drosophila embryos, many biochemically and functionally unrelated transcription factors bind quantitatively to highly overlapping sets of genomic regions, with much of the lowest levels of binding being incidental, non-functional interactions on DNA. The primary biochemical mechanisms that drive these genome-wide occupancy patterns have yet to be established.

Results

Here we use data resulting from the DNaseI digestion of isolated embryo nuclei to provide a biophysical measure of the degree to which proteins can access different regions of the genome. We show that the in vivo binding patterns of 21 developmental regulators are quantitatively correlated with DNA accessibility in chromatin. Furthermore, we find that levels of factor occupancy in vivo correlate mu

SUBMITTER: Li XY 

PROVIDER: S-EPMC3218860 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature