Redefining the relevance of established cancer cell lines to the study of mechanisms of clinical anti-cancer drug resistance.

ABSTRACT: Although in vitro models have been a cornerstone of anti-cancer drug development, their direct applicability to clinical cancer research has been uncertain. Using a state-of-the-art Taqman-based quantitative RT-PCR assay, we investigated the multidrug resistance (MDR) transcriptome of six cancer types, in established cancer cell lines (grown in monolayer, 3D scaffold, or in xenograft) and clinical samples, either containing >75% tumor cells or microdissected. The MDR transcriptome was determined a priori based on an extensive curation of the literature published during the last three decades, which led to the enumeration of 380 genes. No correlation was found between clinical samples and established cancer cell lines. As expected, we found up-regulation of genes that would facilitate survi

SUBMITTER: Gillet JP 

PROVIDER: S-EPMC3219108 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature