Alternative TFAP2A isoforms have distinct activities in breast cancer.

ABSTRACT:

Introduction

AP-2α is a transcription factor implicated in the regulation of differentiation and proliferation in certain tissues, including the mammary gland. In breast tumours, continued expression of AP-2α has been correlated with a better prognosis, but this is hard to reconcile with a reported role in the upregulation of the ERBB2 oncogene. The existence of TFAP2A isoforms, deriving from alternative first exons and differing in their N-terminal sequence, has been described in some mammals, but their relative abundance and activity has not been investigated in the human breast.

Methods

Expression levels of four TFAP2A isoforms were assayed at the level of RNA and protein (via the generation of isoform-specific antibodies) in a panel of breast tumour cell lines and in tis

SUBMITTER: Berlato C 

PROVIDER: S-EPMC3219183 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature