A novel model of common Toll-like receptor 4- and injury-induced transcriptional themes in human leukocytes.

ABSTRACT:

Introduction

An endotoxin challenge, sepsis, and injury/trauma, trigger significant changes in human peripheral blood leukocytes (PBL) gene expression. In this study, we have sought to test the hypothesis that the Toll-like receptor 4 (TLR4) induced transcription patterns elicited in humans exposed to in vivo endotoxin would parallel gene expression patterns observed in trauma patients with initial non-infectious injury. In addition, we sought to identify functional modules that are commonly affected by these two insults of differing magnitude and duration.

Methods

PBL were obtained from seven adult human subject experimental groups. The groups included a group of healthy, hospitalized volunteers (n = 15), that comprised four study groups of subjects challenged with intraven

SUBMITTER: Haimovich B 

PROVIDER: S-EPMC3219281 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature