Microarray analysis of peripheral blood lymphocytes from ALS patients and the SAFE detection of the KEGG ALS pathway.

ABSTRACT:

Background

Sporadic amyotrophic lateral sclerosis (sALS) is a motor neuron disease with poorly understood etiology. Results of gene expression profiling studies of whole blood from ALS patients have not been validated and are difficult to relate to ALS pathogenesis because gene expression profiles depend on the relative abundance of the different cell types present in whole blood. We conducted microarray analyses using Agilent Human Whole Genome 4 × 44k Arrays on a more homogeneous cell population, namely purified peripheral blood lymphocytes (PBLs), from ALS patients and healthy controls to identify molecular signatures possibly relevant to ALS pathogenesis.

Methods

Differentially expressed genes were determined by LIMMA (Linear Models for MicroArray) and SAM (Significance

SUBMITTER: Mougeot JL 

PROVIDER: S-EPMC3219589 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature