Genetic interaction maps in Escherichia coli reveal functional crosstalk among cell envelope biogenesis pathways.

ABSTRACT: As the interface between a microbe and its environment, the bacterial cell envelope has broad biological and clinical significance. While numerous biosynthesis genes and pathways have been identified and studied in isolation, how these intersect functionally to ensure envelope integrity during adaptive responses to environmental challenge remains unclear. To this end, we performed high-density synthetic genetic screens to generate quantitative functional association maps encompassing virtually the entire cell envelope biosynthetic machinery of Escherichia coli under both auxotrophic (rich medium) and prototrophic (minimal medium) culture conditions. The differential patterns of genetic interactions detected among > 235,000 digenic mutant combinations tested reveal unexpected condition-spec

SUBMITTER: Babu M 

PROVIDER: S-EPMC3219608 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature