Postprandial changes in plasma acylcarnitine concentrations as markers of fatty acid flux in overweight and obesity.

ABSTRACT: This study determined whether reductions in postprandial plasma nonesterified fatty acid (FFA) flux would lead to reductions in plasma acylcarnitine (AC) concentrations. Plasma AC was measured by liquid chromatography with tandem mass spectrometry in the fasting state and over 6 hours after a high-fat (50% energy) meal was fed to 16 overweight and obese subjects with a wide range of insulin sensitivities. Body composition was measured by dual-energy x-ray absorptiometry; insulin sensitivity by insulin-modified, frequently sampled intravenous glucose tolerance test; substrate oxidation by indirect calorimetry; blood metabolite and hormone concentrations biochemically; and fatty acid flux by using stable isotope tracers. Lean body mass and fasting fat oxidation correlated positively (r > 0.5

SUBMITTER: Ramos-Roman MA 

PROVIDER: S-EPMC3219812 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature