Age-related changes in relative expression stability of commonly used housekeeping genes in selected porcine tissues.

ABSTRACT:

Background

Gene expression analysis using real-time RT-PCR (qRT-PCR) is increasingly important in biological research due to the high-throughput and accuracy of qRT-PCR. For accurate and reliable gene expression analysis, normalization of gene expression data against housekeeping genes or internal control genes is required. The stability of reference genes has a tremendous effect on the results of relative quantification of gene expression by qRT-PCR. The expression stability of reference genes could vary according to tissues, age of individuals and experimental conditions. In the pig however, very little information is available on the expression stability of reference genes. The aim of this research was therefore to develop a new set of reference genes which can be used for norma

SUBMITTER: Uddin MJ 

PROVIDER: S-EPMC3219825 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature