A proteogenomic analysis of Shigella flexneri using 2D LC-MALDI TOF/TOF.

ABSTRACT:

Background

New strategies for high-throughput sequencing are constantly appearing, leading to a great increase in the number of completely sequenced genomes. Unfortunately, computational genome annotation is out of step with this progress. Thus, the accurate annotation of these genomes has become a bottleneck of knowledge acquisition.

Results

We exploited a proteogenomic approach to improve conventional genome annotation by integrating proteomic data with genomic information. Using Shigella flexneri 2a as a model, we identified total 823 proteins, including 187 hypothetical proteins. Among them, three annotated ORFs were extended upstream through comprehensive analysis against an in-house N-terminal extension database. Two genes, which could not be translated to their full l

SUBMITTER: Zhao L 

PROVIDER: S-EPMC3219829 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature