A comprehensive method for lipid profiling by liquid chromatography-ion cyclotron resonance mass spectrometry.

ABSTRACT: This work aims to combine chromatographic retention, high mass resolution and accuracy, MS/MS spectra, and a package for automated identification and quantitation of lipid species in one platform for lipidomic analysis. The instrumental setup elaborated comprises reversed-phase HPLC coupled to a Fourier transform ion cyclotron resonance mass spectrometer (LTQ-FT), and Lipid Data Analyzer (LDA) software. Data analysis for lipid species quantification in this platform is based on retention time, mass resolution of 200,000, and mass accuracy below 2 ppm. In addition, automatically generated MS/MS spectra provide structural information at molecular level. This LC/MS technology allows analyzing complex biological samples in a quantitative manner as shown here paradigmatically for murine lipid d

SUBMITTER: Fauland A 

PROVIDER: S-EPMC3220297 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature