Single nucleotide polymorphisms that differentiate two subpopulations of Salmonella enteritidis within phage type.

ABSTRACT:

Background

Salmonella Enteritidis is currently the world's leading cause of salmonellosis, in part because of its ability to contaminate the internal contents of eggs. Previous analyses have shown that it is an exceptionally clonal serotype, which nonetheless generates considerable phenotypic heterogeneity. Due to its clonality, whole genome analysis is required to find genetic determinants that contribute to strain heterogeneity of Salmonella Enteritidis. Comparative whole genome mutational mapping of two PT13a strains that varied in the ability to contaminate eggs and to form biofilm was achieved using a high-density tiling platform with primers designed from a PT4 reference genome. Confirmatory Sanger sequencing was used on each putative SNP identified by mutational mapping to c

SUBMITTER: Guard J 

PROVIDER: S-EPMC3220660 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature