Tilt-pair analysis of images from a range of different specimens in single-particle electron cryomicroscopy.

ABSTRACT: The comparison of a pair of electron microscope images recorded at different specimen tilt angles provides a powerful approach for evaluating the quality of images, image-processing procedures, or three-dimensional structures. Here, we analyze tilt-pair images recorded from a range of specimens with different symmetries and molecular masses and show how the analysis can produce valuable information not easily obtained otherwise. We show that the accuracy of orientation determination of individual single particles depends on molecular mass, as expected theoretically since the information in each particle image increases with molecular mass. The angular uncertainty is less than 1° for particles of high molecular mass (~50 MDa), several degrees for particles in the range 1-5 MDa, and tens of

SUBMITTER: Henderson R 

PROVIDER: S-EPMC3220764 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature