Plate reader-based assays for measuring cell viability, neuroprotection and calcium in primary neuronal cultures.

ABSTRACT: Drug discovery and development efforts critically rely on cell-based assays for high-throughput screening. These assay systems mostly utilize immortalized cell lines, such as human embryonic kidney cells, and can provide information on cytotoxicity and cell viability, permeability and uptake of compounds as well as receptor pharmacology. While this approach has proven extremely useful for single-target pharmacology, there is an urgent need for neuropharmacological studies to screen novel drug candidates in a cellular environment resembles neurons in vivo more closely, in order to gain insight into the involvement of multiple signaling pathways. Primary cultured neuronal cells, such as cortical neurons, have long been used for basic research and low-throughput screening and assay developmen

SUBMITTER: Burroughs SL 

PROVIDER: S-EPMC3221776 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature