Structure and stability of RNA/RNA kissing complex: with application to HIV dimerization initiation signal.

ABSTRACT: We develop a statistical mechanical model to predict the structure and folding stability of the RNA/RNA kissing-loop complex. One of the key ingredients of the theory is the conformational entropy for the RNA/RNA kissing complex. We employ the recently developed virtual bond-based RNA folding model (Vfold model) to evaluate the entropy parameters for the different types of kissing loops. A benchmark test against experiments suggests that the entropy calculation is reliable. As an application of the model, we apply the model to investigate the structure and folding thermodynamics for the kissing complex of the HIV-1 dimerization initiation signal. With the physics-based energetic parameters, we compute the free energy landscape for the HIV-1 dimer. From the energy landscape, we identify two

SUBMITTER: Cao S 

PROVIDER: S-EPMC3222126 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature