Molecular analysis of T-DNA insertion mutants identified putative regulatory elements in the AtTERT gene.

ABSTRACT: Analysis of plants bearing a T-DNA insertion is a potent tool of modern molecular biology, providing valuable information about the function and involvement of genes in metabolic pathways. A collection of 12 Arabidopsis thaliana lines with T-DNA insertions in the gene coding for the catalytic subunit of telomerase (AtTERT) and in adjacent regions was screened for telomerase activity [telomere repeat amplification protocol (TRAP) assay], telomere length (terminal restriction fragments), and AtTERT transcription (quantitative reverse transcription-PCR). Lines with the insertion located upstream of the start codon displayed unchanged telomere stability and telomerase activity, defining a putative minimal AtTERT promoter and the presence of a regulatory element linked to increased transcriptio

SUBMITTER: Fojtova M 

PROVIDER: S-EPMC3223050 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature