Assessment of hypoxia inducible factor levels in cancer cell lines upon hypoxic induction using a novel reporter construct.

ABSTRACT: Hypoxia Inducible Factor (HIF) signaling pathway is important for tumor cells with limited oxygen supplies, as it is shown to be involved in the process of proliferation and angiogenesis. Given its pivotal role in cancer biology, robust assays for tracking changes in HIF expression are necessary for understanding its regulation in cancer as well as developing therapies that target HIF signaling. Here we report a novel HIF reporter construct containing tandem repeats of minimum HIF binding sites upstream of eYFP coding sequence. We show that the reporter construct has an excellent signal to background ratio and the reporter activity is HIF dependent and directly correlates with HIF protein levels. By utilizing this new construct, we assayed HIF activity levels in different cancer cell lines

SUBMITTER: Zhou W 

PROVIDER: S-EPMC3223176 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature