Synergy analysis reveals association between insulin signaling and desmoplakin expression in palmitate treated HepG2 cells.

ABSTRACT: The regulation of complex cellular activities in palmitate treated HepG2 cells, and the ensuing cytotoxic phenotype, involves cooperative interactions between genes. While previous approaches have largely focused on identifying individual target genes, elucidating interacting genes has thus far remained elusive. We applied the concept of information synergy to reconstruct a "gene-cooperativity" network for palmititate-induced cytotoxicity in liver cells. Our approach integrated gene expression data with metabolic profiles to select a subset of genes for network reconstruction. Subsequent analysis of the network revealed insulin signaling as the most significantly enriched pathway, and desmoplakin (DSP) as its top neighbor. We determined that palmitate significantly reduces DSP expression,

SUBMITTER: Wang X 

PROVIDER: S-EPMC3223234 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature