A novel fungal family of oligopeptide transporters identified by functional metatranscriptomics of soil eukaryotes.

ABSTRACT: Functional environmental genomics has the potential to identify novel biological functions that the systematic sequencing of microbial genomes or environmental DNA may fail to uncover. We targeted the functions expressed by soil eukaryotes using a metatranscriptomic approach based on the use of soil-extracted polyadenylated messenger RNA to construct environmental complementary DNA expression libraries. Functional complementation of a yeast mutant defective in di/tripeptide uptake identified a novel family of oligopeptide transporters expressed by fungi. This family has a patchy distribution in the Basidiomycota and Ascomycota and is present in the genome of a Saccharomyces cerevisiae wine strain. High throughput phenotyping of yeast mutants expressing two environmental transporters showed

SUBMITTER: Damon C 

PROVIDER: S-EPMC3223307 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature