Genome-wide functional annotation and structural verification of metabolic ORFeome of Chlamydomonas reinhardtii.

ABSTRACT:

Background

Recent advances in the field of metabolic engineering have been expedited by the availability of genome sequences and metabolic modelling approaches. The complete sequencing of the C. reinhardtii genome has made this unicellular alga a good candidate for metabolic engineering studies; however, the annotation of the relevant genes has not been validated and the much-needed metabolic ORFeome is currently unavailable. We describe our efforts on the functional annotation of the ORF models released by the Joint Genome Institute (JGI), prediction of their subcellular localizations, and experimental verification of their structural annotation at the genome scale.

Results

We assigned enzymatic functions to the translated JGI ORF models of C. reinhardtii by reciprocal BLAS

SUBMITTER: Ghamsari L 

PROVIDER: S-EPMC3223727 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature