Filtering "genic" open reading frames from genomic DNA samples for advanced annotation.

ABSTRACT:

Background

In order to carry out experimental gene annotation, DNA encoding open reading frames (ORFs) derived from real genes (termed "genic") in the correct frame is required. When genes are correctly assigned, isolation of genic DNA for functional annotation can be carried out by PCR. However, not all genes are correctly assigned, and even when correctly assigned, gene products are often incorrectly folded when expressed in heterologous hosts. This is a problem that can sometimes be overcome by the expression of protein fragments encoding domains, rather than full-length proteins. One possible method to isolate DNA encoding such domains would to "filter" complex DNA (cDNA libraries, genomic and metagenomic DNA) for gene fragments that confer a selectable phenotype relying on cor

SUBMITTER: D'Angelo S 

PROVIDER: S-EPMC3223728 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature