Proteomic analysis of the carotenogenic yeast Xanthophyllomyces dendrorhous.

ABSTRACT:

Background

The yeast Xanthophyllomyces dendrorhous is used for the microbiological production of the antioxidant carotenoid astaxanthin. In this study, we established an optimal protocol for protein extraction and performed the first proteomic analysis of the strain ATCC 24230. Protein profiles before and during the induction of carotenogenesis were determined by two-dimensional polyacrylamide gel electrophoresis and proteins were identified by mass spectrometry.

Results

Among the approximately 600 observed protein spots, 131 non-redundant proteins were identified. Proteomic analyses allowed us to identify 50 differentially expressed proteins that fall into several classes with distinct expression patterns. These analyses demonstrated that enzymes related to acetyl-CoA synth

SUBMITTER: Martinez-Moya P 

PROVIDER: S-EPMC3224108 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature