The protein interaction map of bacteriophage lambda.

ABSTRACT:

Background

Bacteriophage lambda is a model phage for most other dsDNA phages and has been studied for over 60 years. Although it is probably the best-characterized phage there are still about 20 poorly understood open reading frames in its 48-kb genome. For a complete understanding we need to know all interactions among its proteins. We have manually curated the lambda literature and compiled a total of 33 interactions that have been found among lambda proteins. We set out to find out how many protein-protein interactions remain to be found in this phage.

Results

In order to map lambda's interactions, we have cloned 68 out of 73 lambda open reading frames (the "ORFeome") into Gateway vectors and systematically tested all proteins for interactions using exhaustive array-based

SUBMITTER: Rajagopala SV 

PROVIDER: S-EPMC3224144 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature