Epigenetic modification does not determine the time of POU5F1 transcription activation in cloned bovine embryos.

ABSTRACT:

Purpose

To investigate the effect of epigenetic modification on pattern, time and capacity of transcription activation of POU5F1, the key marker of pluripotency, in cloned bovine embryos.

Methods

Bovine fibroblasts were stably transfected with POU5F1 promoter-driven enhanced green fluorescent protein (EGFP). This provided a visible marker to investigate the effect of post-activation treatment of cloned bovine embryos with trichostatin A (TSA) on time and capacity of POU5F1 expression and its subsequent effect on in vitro development of cloned bovine embryos.

Results

Irrespective of TSA treatment, POU5F1 expression was not detected until 8-16 cell stage, but was detected in both inner cell mass and trophectoderm at the blastocyst stage. TSA treatment significantly incr

SUBMITTER: Jafari S 

PROVIDER: S-EPMC3224165 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature