Global gene expression profiling of Plasmodium falciparum in response to the anti-malarial drug pyronaridine.

ABSTRACT:

Background

Pyronaridine (PN) and chloroquine (CQ) are structurally related anti-malarial drugs with primarily the same mode of action. However, PN is effective against several multidrug-resistant lines of Plasmodium falciparum, including CQ resistant lines, suggestive of important operational differences between the two drugs.

Methods

Synchronized trophozoite stage cultures of P. falciparum strain K1 (CQ resistant) were exposed to 50% inhibitory concentrations (IC50) of PN and CQ, and parasites were harvested from culture after 4 and 24 hours exposure. Global transcriptional changes effected by drug treatment were investigated using DNA microarrays.

Results

After a 4 h drug exposure, PN induced a greater degree of transcriptional perturbation (61 differentially expres

SUBMITTER: Kritsiriwuthinan K 

PROVIDER: S-EPMC3224238 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature