A comprehensive transcriptome and immune-gene repertoire of the lepidopteran model host Galleria mellonella.

ABSTRACT:

Background

The larvae of the greater wax moth Galleria mellonella are increasingly used (i) as mini-hosts to study pathogenesis and virulence factors of prominent bacterial and fungal human pathogens, (ii) as a whole-animal high throughput infection system for testing pathogen mutant libraries, and (iii) as a reliable host model to evaluate the efficacy of antibiotics against human pathogens. In order to compensate for the lack of genomic information in Galleria, we subjected the transcriptome of different developmental stages and immune-challenged larvae to next generation sequencing.

Results

We performed a Galleria transcriptome characterization on the Roche 454-FLX platform combined with traditional Sanger sequencing to obtain a comprehensive transcriptome. To maximize se

SUBMITTER: Vogel H 

PROVIDER: S-EPMC3224240 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature