Identification, structure, and characterization of an exopolysaccharide produced by Histophilus somni during biofilm formation.

ABSTRACT:

Background

Histophilus somni, a gram-negative coccobacillus, is an obligate inhabitant of bovine and ovine mucosal surfaces, and an opportunistic pathogen responsible for respiratory disease and other systemic infections in cattle and sheep. Capsules are important virulence factors for many pathogenic bacteria, but a capsule has not been identified on H. somni. However, H. somni does form a biofilm in vitro and in vivo, and the biofilm matrix of most bacteria consists of a polysaccharide.

Results

Following incubation of H. somni under growth-restricting stress conditions, such as during anaerobiosis, stationary phase, or in hypertonic salt, a polysaccharide could be isolated from washed cells or culture supernatant. The polysaccharide was present in large amounts in broth cu

SUBMITTER: Sandal I 

PROVIDER: S-EPMC3224263 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature