Comprehensive transcriptome analysis of the highly complex Pisum sativum genome using next generation sequencing.

ABSTRACT:

Background

The garden pea, Pisum sativum, is among the best-investigated legume plants and of significant agro-commercial relevance. Pisum sativum has a large and complex genome and accordingly few comprehensive genomic resources exist.

Results

We analyzed the pea transcriptome at the highest possible amount of accuracy by current technology. We used next generation sequencing with the Roche/454 platform and evaluated and compared a variety of approaches, including diverse tissue libraries, normalization, alternative sequencing technologies, saturation estimation and diverse assembly strategies. We generated libraries from flowers, leaves, cotyledons, epi- and hypocotyl, and etiolated and light treated etiolated seedlings, comprising a total of 450 megabases. Libraries were

SUBMITTER: Franssen SU 

PROVIDER: S-EPMC3224338 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature